Specific high-affinity binding of fatty acids to epidermal cytosolic proteins

Journal Article · Thu Aug 01 00:00:00 EDT 1991 · Journal of Investigative Dermatology; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12480586· OSTI ID:5323447

Raza, H; Chung, W L; Mukhtar, H ^[1]

---

1. Department of Dermatology, University Hospitals of Cleveland, Case Western Reserve University, OH (USA)

Cytosol from rat, mouse, and human skin or rat epidermis was incubated with (3H)arachidonic acid, (14C)retinoic acid, (14C)oleic acid, (3H)leukotriene A4, (3H)prostaglandin E2 (PGE2) or (3H) 15-hydroxyeicosatetraenoic acid (15-HETE), and protein-bound ligands were separated using Lipidex-1000 at 4C to assess the binding specificity. The binding of oleic acid and arachidonic acid with rat epidermal cytosol was rapid, saturable, and reversible. Binding of oleic acid was competed out with the simultaneous addition of other ligands and found to be in the following order: arachidonic acid greater than oleic acid greater than linoleic acid greater than lauric acid greater than leukotriene A4 greater than 15-HETE = PGE1 greater than PGE2 = PGF2. Scatchard analysis of the binding with arachidonic acid, oleic acid, and retinoic acid revealed high-affinity binding sites with the dissociation constant in the nM range. SDS-PAGE analysis of the oleic acid-bound epidermal cytosolic protein(s) revealed maximum binding at the 14.5 kDa region. The presence of the fatty acid-binding protein in epidermal cytosol and its binding to fatty acids and retinoic acid may be of significance both in the trafficking and the metabolism of fatty acids and retinoids across the skin.

• OSTI ID: 5323447
• Journal Information: Journal of Investigative Dermatology; (United States), Vol. 97:2; ISSN 0022-202X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
BIOCHEMICAL REACTION KINETICS
AFFINITY
ARACHIDONIC ACID
CARBON 14 COMPOUNDS
ELECTROPHORESIS
EPIDERMIS
LIGANDS
MAN
METABOLISM
MICE
OLEIC ACID
PROSTAGLANDINS
RATS
RECEPTORS
RETINOIC ACID
SKIN
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL TISSUES
ANIMALS
BODY
CARBON COMPOUNDS
CARBOXYLIC ACID ESTERS
EPITHELIUM
ESTERS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
REACTION KINETICS
RODENTS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques