Aggregation of macrophages and fibroblasts is inhibited by a monoclonal antibody to the hyaluronate receptor

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90393-X· OSTI ID:5322223

Green, S J; Underhill, C B ^[1]; Tarone, G ^[2]

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1. Georgetown Univ. Medical Center, Washington, DC (USA)
2. Univ. of Turin (Italy)

To examine the role of the hyaluronate receptor in cell to cell adhesion, the authors have employed the K-3 monoclonal antibody (MAb) which specifically binds to the hyaluronate receptor and blocks its ability to interact with hyaluronate. In the first set of experiments, they investigated the spontaneous aggregation of SV-3T3 cells, which involves two distinct mechanisms, one of which is dependent upon the presence of divalent cation and the other is independent. The divalent cation-independent aggregation was found to be completely inhibited by both intact and Fab fragments of the K-3 MAb. In contrast, the K-3 MAb had no effect on the divalent cation-dependent aggregation of cells. In a second set of experiments, we examined alveolar macrophages. The presence of hyaluronate receptors on alveolar macrophages was demonstrated by the fact that detergent extracts of these cells could bind ({sup 3})hyaluronate, and this binding was blocked by the K-3 MAb. Immunoblot analysis of alveolar macrophages showed that the hyaluronate receptor had a M{sub r} of 99,500, which is considerably larger than the 85,000 M{sub r} for that on BHK cells. When hyaluronate was added to suspensions of alveolar macrophages, the cells were induced to aggregate. This effect was inhibited by the K-3 MAb, suggesting that the hyaluronate-induced aggregation was mediated by the receptor.

• OSTI ID: 5322223
• Journal Information: Experimental Cell Research; (United States), Vol. 178:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FIBROBLASTS
AGGLOMERATION
HYALURONIC ACID
RADIORECEPTOR ASSAY
MACROPHAGES
MONOCLONAL ANTIBODIES
CROSS-LINKING
RECEPTORS
ADHESION
BIOCHEMICAL REACTION KINETICS
HAMSTERS
IMMUNOGLOBULINS
INHIBITION
LUNGS
MICE
TRITIUM COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
ANTIBODIES
BODY
CARBOHYDRATES
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
GLOBULINS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
POLYMERIZATION
POLYSACCHARIDES
PROTEINS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES
550301* - Cytology- Tracer Techniques