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Title: Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells

Abstract

Protein C23 (also called nucleolin or 100-kDa nucleolar protein) is a major nucleolar phosphoprotein involved in ribosome biogenesis. To determine the effects of protein C23 on preribosomal RNA (pre-rRNA) synthesis anti-C23 antiserum was microinjected into nuclei of Chironomus tentans salivary glands. Transcription was measured by incubation of the glands with {sup 32}P-labeled RNA precursors followed by microdissection of nucleoli, RNA extraction, and electrophoretic analyses. Injection of the anti-C23 antibody caused a 2- to 3.5-fold stimulation of {sup 32}P incorporation into 38 S pre-rRNA. No stimulation was observed in salivary glands injected with preimmune serum or antiserum preabsorbed with protein C23. The stimulatory effect was selective for pre-rRNA as indicated by the lack of stimulation of {sup 32}P incorporation into extranucleolar RNA. Injection of the antiserum produced little or no effect on pre-RNA processing as measured by the relative amounts of {sup 32}P-labeled intermediate cleavage products of pre-rRNA in stimulated versus control glands. These results suggest that protein C23 not only is involved in ribosome assembly but also plays a role in regulating the transcription of the preribosomal RNA.

Authors:
;  [1]; ; ; ; ; ;  [2]
  1. Karolinska Institutet, Stockholm (Sweden)
  2. Univ. of Mississippi Medical Center, Jackson (USA)
Publication Date:
OSTI Identifier:
5321997
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research; (United States)
Additional Journal Information:
Journal Volume: 178:2; Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; NUCLEOPROTEINS; BIOCHEMISTRY; RIBOSOMAL RNA; BIOSYNTHESIS; ANTIBODIES; INSECTS; PHOSPHORUS 32; RIBOSOMES; SALIVARY GLANDS; TRANSCRIPTION; ANIMALS; ARTHROPODS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CELL CONSTITUENTS; CHEMISTRY; DAYS LIVING RADIOISOTOPES; GLANDS; INVERTEBRATES; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; RNA; SYNTHESIS; 550401* - Genetics- Tracer Techniques

Citation Formats

Egyhazi, E, Pigon, A, Chang, Jinhong, Ghaffari, S H, Dreesen, T D, Wellman, S E, Case, S T, and Olson, M O.J. Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells. United States: N. p., 1988. Web. doi:10.1016/0014-4827(88)90397-7.
Egyhazi, E, Pigon, A, Chang, Jinhong, Ghaffari, S H, Dreesen, T D, Wellman, S E, Case, S T, & Olson, M O.J. Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells. United States. https://doi.org/10.1016/0014-4827(88)90397-7
Egyhazi, E, Pigon, A, Chang, Jinhong, Ghaffari, S H, Dreesen, T D, Wellman, S E, Case, S T, and Olson, M O.J. 1988. "Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells". United States. https://doi.org/10.1016/0014-4827(88)90397-7.
@article{osti_5321997,
title = {Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells},
author = {Egyhazi, E and Pigon, A and Chang, Jinhong and Ghaffari, S H and Dreesen, T D and Wellman, S E and Case, S T and Olson, M O.J.},
abstractNote = {Protein C23 (also called nucleolin or 100-kDa nucleolar protein) is a major nucleolar phosphoprotein involved in ribosome biogenesis. To determine the effects of protein C23 on preribosomal RNA (pre-rRNA) synthesis anti-C23 antiserum was microinjected into nuclei of Chironomus tentans salivary glands. Transcription was measured by incubation of the glands with {sup 32}P-labeled RNA precursors followed by microdissection of nucleoli, RNA extraction, and electrophoretic analyses. Injection of the anti-C23 antibody caused a 2- to 3.5-fold stimulation of {sup 32}P incorporation into 38 S pre-rRNA. No stimulation was observed in salivary glands injected with preimmune serum or antiserum preabsorbed with protein C23. The stimulatory effect was selective for pre-rRNA as indicated by the lack of stimulation of {sup 32}P incorporation into extranucleolar RNA. Injection of the antiserum produced little or no effect on pre-RNA processing as measured by the relative amounts of {sup 32}P-labeled intermediate cleavage products of pre-rRNA in stimulated versus control glands. These results suggest that protein C23 not only is involved in ribosome assembly but also plays a role in regulating the transcription of the preribosomal RNA.},
doi = {10.1016/0014-4827(88)90397-7},
url = {https://www.osti.gov/biblio/5321997}, journal = {Experimental Cell Research; (United States)},
issn = {0014-4827},
number = ,
volume = 178:2,
place = {United States},
year = {Sat Oct 01 00:00:00 EDT 1988},
month = {Sat Oct 01 00:00:00 EDT 1988}
}