Enzymatic amplification of a Y chromosome repeat in a single blastomere allows identification of the sex of preimplantation mouse embryos
- Mount Sinai School of Medicine, New York, NY (USA)
The polymerase chain reaction (PCR) technique has been adapted to identify the sex of preimplantation mouse embryos rapidly. PCR was used to amplify a specific repeated DNA sequence on the Y chromosome from a single isolated blastomere in under 12 hr. The remainder of the biopsied embryo was then transferred to a pseudopregnant female and carried to term. Using this technique, 72% of embryos can be classed as potentially either male or female. Transfers of such embryos have produced pregnancies with 8/8 fetuses (100%) being of the predicted sex. Variations of the technique have demonstrated certain limitations to the present procedure as well as indicated possible strategies for improvement of the assay. The PCR technique may have wide application in the genetic analysis of preimplantation embryos.
- OSTI ID:
- 5321986
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:11; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
Similar Records
Chromosome abnormalities in human arrested preimplantation embryos: A multiple-probe FISH study
Pre-implantation diagnosis of aneuploidy by polar body and blastomere FISH analysis
Related Subjects
DNA POLYMERASES
BIOCHEMISTRY
EMBRYOS
SEX
Y CHROMOSOME
GENE AMPLIFICATION
DNA HYBRIDIZATION
DNA SEQUENCING
MICE
OLIGONUCLEOTIDES
PHOSPHORUS 32
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMISTRY
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
ENZYMES
HETEROCHROMOSOMES
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOISOTOPES
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
550401* - Genetics- Tracer Techniques