Altered polymorphonuclear leukocyte size in iron deficient rats

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5321644

Miller, R; Sherman, A R

Iron deficiency (-Fe) in rat pups impairs cellular growth of immunocompetent organs and phagocytosis. To determine if peripheral blood polymorphnuclear leukocytes (PMNL) size is altered, DNA and protein (PRO) were measured in PMNL during -Fe and repletion pre- and post-weaning. Dams (n = 12) were fed 6 ppm Fe (D) or 250 ppm Fe (C) from dl pregnancy until d21 lactation. Litters were adjusted to 7 pups. On d17, 1 pup/litter was used for PMNL PRO and DNA determinations. Littermates were weaned on d21 to either the same diet fed to their dams (DD,CC); repleted with Fe (DC); or fed-Fe diet (CD), until days 44, 63, and 84. PMNL were separated on a discontinuous Percoll density gradient. D17 pups of D dams had lower Hb vs C (p < .01). Littermates weaned to 6 ppm Fe (DD,CC) had lower Hb levels compared to C groups (CC,DD) postweaning (p < .01). PMNL PRO and DNA in D pups were one-half those in C pups (p < .01) on d17, however PRO:DNA was increased (p < .05). Pups repleted (DC) at d44 , 63 and 84 had increased PMNL PRO vs DD (p < .01). Diminished PMNL PRO was seen in CD pups on d44, 63 and 84. Fe repletion (DC) increased PMNL DNA 2-fold vs DD from d44 on. -Fe diet caused a marked depletion in PMNL DNA in CD vs CC at all postweaning ages. PRO:DNA in DD and CD groups shows a significant increase in PMNL size. Fe repletion (DC) reduced the hypertrophy of PMNL.

• Research Organization: Univ. of Illinois, Urbana
• OSTI ID: 5321644
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
IRON
NUTRITIONAL DEFICIENCY
TOXICITY
LEUKOCYTES
SIZE
DIET
DNA
LACTATION
PREGNANCY
RATS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
ELEMENTS
MAMMALS
MATERIALS
METALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RODENTS
TRANSITION ELEMENTS
VERTEBRATES
560305* - Chemicals Metabolism &amp; Toxicology- Vertebrates- (-1987)