Opiate antagonist binding sites in discrete brain regions of spontaneously hypertensive and normotensive Wistar-Kyoto rats

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(91)90604-A· OSTI ID:5316953

Rahmani, N H; Gulati, A; Bhargava, H N ^[1]

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1. Univ. of Illinois, Chicago (USA)

The binding of {sup 3}H-naltrexone, an opiate receptor antagonist, to membranes of discrete brain regions and spinal cord of 10 week old spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats was determined. The brain regions examined were hypothalamus, amygdala, hippocampus, corpus striatum, pons and medulla, midbrain and cortex. {sup 3}H-Naltrexone bound to membranes of brain regions and spinal cord at a single high affinity site with an apparent dissociation constant value of 3 nM. The highest density of {sup 3}H-naltrexone binding sites were in hippocampus and lowest in the cerebral cortex. The receptor density (B{sub max}value) and apparent dissociation constant (K{sub d} value) values of {sup 3}H-naltrexone to bind to opiate receptors on the membranes of amygdala, hippocampus, corpus striatum, pons and medulla, midgrain, cortex and spinal cord of WKY and SHR rates did not differ. The B{sub max} value of {sup 3}H-naltrexone binding to membranes of hypothalamus of SHR rates was 518% higher than WKY rats but the K{sub d} values in the two strains did not differ. It is concluded that SHR rats have higher density of opiate receptors labeled with {sup 3}H-naltrexone in the hypothalamus only, in comparison with WKY rats, and that such a difference in the density of opiate receptors may be related to the elevated blood pressure in SHR rats.

• OSTI ID: 5316953
• Journal Information: Life Sciences; (United States), Vol. 48:26; ISSN 0024-3205
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANTIMETABOLITES
BIOCHEMICAL REACTION KINETICS
ENDORPHINS
RECEPTORS
HYPERTENSION
PATHOGENESIS
AFFINITY
BLOOD PRESSURE
BRAIN
CELL MEMBRANES
GENETIC VARIABILITY
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RATS
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BODY
CARDIOVASCULAR DISEASES
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
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DRUGS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RODENTS
SYMPTOMS
VASCULAR DISEASES
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