Stimulation of polyunsaturated fatty acid oxidation in myocytes by regulating its cellular uptake

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(91)90002-S· OSTI ID:5316766

Abdel-aleem, S; Frangakis, C ^[1]; Badr, M ^[2]

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1. Glaxo Inc., Research Triangle Park, NC (USA)
2. Univ. of Missouri-Kansas City, MO (USA)

In order to investigate the regulation of polyunsaturated fatty acid oxidation in the heart, the effect of the phosphodiesterase inhibitor enoximone on the oxidation of (1-{sup 14}C) arachidonic acid, and (1-{sup 14}C) arachidonyl-CoA, were studied in adult rat myocytes, and isolated rat heart mitochondria. Enoximone stimulated arachidonate oxidation by 94%, at a concentration of 0.25 mM. The apparent Vmax value of archidonate oxidation in the presence of enoximone was approximately 75% higher than the value observed with the control in isolated myocytes. Also, enoximone stimulated arachidonate uptake by 27% at a concentration of 0.25 mM. On the other hand, enoximone had no effect on the oxidation of (1-{sup 14}C) arachidonyl-CoA in isolated rat heart mitochondria. These results suggest that the oxidation of polyunsaturated fatty acids in myocytes is regulated by the rate of uptake of these acids across sarcolemmal membranes.

• OSTI ID: 5316766
• Journal Information: Life Sciences; (United States), Vol. 49:3; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
METABOLISM
ENZYME INHIBITORS
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CELL MEMBRANES
HEART
MEMBRANE TRANSPORT
MITOCHONDRIA
TRACER TECHNIQUES
UPTAKE
BODY
CARBON COMPOUNDS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
550501* - Metabolism- Tracer Techniques