Estrogen retention and estrogen receptor distribution in uterus of rats deficient in zinc and/or vitamin B/sub 6/
Holley et al have reported that uptake and retention of a tracer dose of (/sup 3/H)-estradiol (E/sub 2/) by rat uteri nuclei was increased four-fold in pyridoxine-deprived young rats as compared to controls. The diet lacked a specific input of zinc, a nutrient which may also influence estrogen impact on target cells. The authors have tested the effect of diets restricted in either zinc or pyridoxine singly or in combination upon both retention of estrogen and subcellular distribution of estrogen receptor in rat uterus. Female Sprague-Dawley rats were fed their respective diets for five weeks. Stage of estrous cycle was determined by examination of vaginal smears. On the morning of estrous, each rat was given an IP injection of (/sup 3/H) E/sub 2/. Nuclear and cytosolic E/sub 2/ was determined after 20 minutes. A second series of animals were killed at estrous after the same period of dietary treatment and nuclear and cytosolic estradiol receptors were measured. Uterine retention of injected E/sub 2/ was increased 2-fold when Zn was limiting (3 ppm), 1.5-fold when B/sub 6/ was low and 3.5-fold when both were low. Dually deficient rats displayed a 10-fold increase in nuclear content of E/sub 2/ receptor but no significant change in total cellular receptor content.
- Research Organization:
- Virginia Tech, Blacksburg
- OSTI ID:
- 5316205
- Report Number(s):
- CONF-8604222-; TRN: 86-028584
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ESTROGENS
RECEPTORS
RETENTION
PYRIDOXINE
NUTRITIONAL DEFICIENCY
ZINC
ESTROUS CYCLE
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UTERUS
ANIMALS
AZINES
BODY
ELEMENTS
FEMALE GENITALS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
METALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIDINES
RODENTS
STEROID HORMONES
VERTEBRATES
VITAMIN B GROUP
VITAMINS
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550201 - Biochemistry- Tracer Techniques