Distinct angiotensin II receptor in primary cultures of glial cells from rat brain

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.13.4655· OSTI ID:5315449

Raizada, M K; Phillips, M I; Crews, F T; Sumners, C

Angiotensin II (Ang-II) has profound effects on the brain. Receptors for Ang-II have been demonstrated on neurons, but no relationship between glial cells and Agn-II has been established. Glial cells (from the hypothalamus and brain stem of 1-day-old rat brains) in primary culture have been used to demonstrate the presence of specific Ang-II receptors. Binding of /sup 125/I-Ang-II to glial cultures was rapid, reversible, saturable, and specific for Ang-II. The rank order of potency of /sup 125/I-Ang-II binding was determined. Scatchard analysis revealed a homogeneous population of high-affinity binding sites with a B/sub max/ of 110 fmol/mg of protein. Light-microscopic autoradiography of /sup 125/I-Ang-II binding supported the kinetic data, documenting specific Ang-II receptors on the glial cells. Ang-II stimulated a dose-dependent hydrolysis of phosphatidylinositols in glial cells, an effect mediated by Ang-II receptors. However, Ang-II failed to influence (/sup 3/H) norepinephrine uptake, and catecholamines failed to regulate Ang-II receptors, effects that occur in neurons. These observations demonstrate the presence of specific Ang-II receptors on the glial cells in primary cultures derived from normotensive rat brain. The receptors are kinetically similar to, but functionally distinct from, the neuronal Ang-II receptors.

• Research Organization: Univ. of Florida, Gainesville (USA)
• OSTI ID: 5315449
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:13
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
ANGIOTENSIN
CONFIGURATION INTERACTION
LABELLED COMPOUNDS
NERVE CELLS
AUTORADIOGRAPHY
RECEPTORS
BIOCHEMICAL REACTION KINETICS
BRAIN
CELL CULTURES
DOSE-RESPONSE RELATIONSHIPS
NORADRENALINE
RATS
TRITIUM COMPOUNDS
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
DRUGS
GLOBULINS
HORMONES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RODENTS
SOMATIC CELLS
STEROID HORMONES
SYMPATHOMIMETICS
VASOCONSTRICTORS
VERTEBRATES
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