Regional changes in the binding of (/sup 3/H) concanavalin A to mouse blastocysts at implantation: an autoradiographic study

Journal Article · Sun Nov 01 00:00:00 EST 1981 · J. Exp. Zool.; (United States)

DOI:https://doi.org/10.1002/jez.1402180217· OSTI ID:5311090

Carollo, J R; Weitlauf, H M

Implanting and delayed-implanting mouse blastocysts were incubated in vitro with (3H) concanavalin A (Con A), and the distribution of binding on their surfaces was determined by light microscopic autoradiography. The density of binding was uniform on the trophectoderm of delayed-implanting embryos and was not changed on the polar surface of implanting embryos. However, binding was reduced on the proximal mural and distal mural trophectoderm of implanting blastocysts by 36% and 60%, respectively. These results suggest that there is a regional reduction in the density of mannose-like sugars on the surface of mouse blastocysts at the time of attachment and implantation.

• Research Organization: Department of Anatomy, School of Medicine, University of Oregon Health Sciences Center, Portland, Oregon
• OSTI ID: 5311090
• Journal Information: J. Exp. Zool.; (United States), Vol. 218:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CONCANAVALIN
BIOCHEMICAL REACTION KINETICS
EMBRYONIC CELLS
PHYSIOLOGY
TRITIUM COMPOUNDS
ISOTOPE APPLICATIONS
AUTORADIOGRAPHY
EMBRYOS
IN VITRO
OPTICAL MICROSCOPY
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
HEMAGGLUTININS
KINETICS
LABELLED COMPOUNDS
MICROSCOPY
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques