Catecholamine regulation of lactate dehydrogenase in rat brain cell culture
The mechanism of catecholamine induction of the soluble cytoplasmic enzyme lactate dehydrogenase (EC 1.1.1.27) was studied in the rat glial tumor cell line, C6. Lactate dehydrogenase was partially purified from extracts of (/sup 3/H)leucine-labeled cells by affinity gel chromatography and quantitatively immunoprecipitated with anti-lactate dehydrogenase-5 IgG and with antilactate dehydrogenase-1 IgG. The immunoprecipitates were dissociated and electrophoresed on sodium dodecyl sulfate polyacrylamide gels. Using this methodology, the increased enzyme activity of lactate dehydrogenase in norepinephrine-treated C6 cells was observed to be concomitant with the increased synthesis of enzyme molecules. Despite the continued presence of norepinephrine, the specific increase in the rate of synthesis of lactate dehydrogenase was transient. It was first detected at 4 h, was maximum at 9 h, and returned to basal levels by 24 h. The half-life of lactate dehydrogenase enzyme activity was 36 h during the induction and 40 h during deinduction. The half-life for decay of /sup 3/H-labeled lactate dehydrogenase was 41 h. These observations suggest that the increase in lactate dehydrogenase activity in norepinephrine-treated cells does not involve any change in the rate of degradation. Norepinephrine increased the specific rate of synthesis of both lactate dehydrogenase-5 (a tetramer of four M subunits) and lactate dehydrogenase-1 (a tetramer of four H subunits), although to different extents. Since these subunits are coded for by two separate genes on separate chromosomes, it suggests that the regulatory mechanism involves at least two separate sites of action.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 5308437
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 255:6
- Country of Publication:
- United States
- Language:
- English
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LACTATE DEHYDROGENASE
ENZYME ACTIVITY
NORADRENALINE
BIOLOGICAL EFFECTS
BIOCHEMICAL REACTION KINETICS
BRAIN
CELL CULTURES
GENES
LABELLED COMPOUNDS
RATS
TRITIUM
TUMOR CELLS
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CENTRAL NERVOUS SYSTEM
DEHYDROGENASES
DRUGS
ENZYMES
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HYDROGEN ISOTOPES
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OXIDOREDUCTASES
RADIOISOTOPES
REACTION KINETICS
RODENTS
STEROID HORMONES
SYMPATHOMIMETICS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550200* - Biochemistry
550300 - Cytology