Bacteriophage T5 transfer RNA

Journal Article · Thu Apr 10 00:00:00 EST 1980 · J. Biol. Chem.; (United States)

OSTI ID:5307269

Hunt, C; Desai, S M; Vaughan, J; Weiss, S B

Previous studies from this laboratory have provided a high resolution map for 16 tRNA genes located on the continuous heavy DNA strand of bacteriophage T5 DNA. All of the T5 tRNA genes were located in three clusters within the DNA C segment, except for tRNA/sup Arg/, which mapped on the left end of the DNA D segment. In this report, we present evidence for the presence of eight additional T5 tRNA species, five of which are located in two new loci within the DNA C segment. We also describe a two-dimensional gel electrophoresis system for the separation and isolation of T5 tRNA species from crude infected RNA preparations. The gel electrophoresis system separates tRNA isoacceptors specific for different amino acids; evidence is presented that the isoacceptors for isoleucine, histidine, and serine are coded by different T5 genes.

• Research Organization: Univ. of Chicago, IL
• DOE Contract Number: EY-76-C-02-0069
• OSTI ID: 5307269
• Journal Information: J. Biol. Chem.; (United States), Vol. 255:7
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
TRANSFER RNA
GENETIC MAPPING
BACTERIOPHAGES
DNA
ELECTROPHORESIS
GENES
HISTIDINE
SERINE
TWO-DIMENSIONAL CALCULATIONS
AMINO ACIDS
AZOLES
CARBOXYLIC ACIDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
IMIDAZOLES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
RNA
VIRUSES
550400* - Genetics
550200 - Biochemistry
550700 - Microbiology