Cytotoxic and mutagenic properties of shale oil byproducts. I. Activation of retort process waters with near ultraviolet light
Cultured Chinese hamster ovary (CHO) cells were exposed to dilutions of shale oil retort process waters obtained from three different retorting processes located in the Green River oil shale formations in the western part of the United States. Although the intensity of the response was dictated by the process water used, all induced a cytotoxic (reduction in colony-forming ability) and mutagenic (induced at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus) response in cells pretreated with dilutions of the waters and subsequent exposure to near ultraviolet light (NUV). Combinations of process water plus NUV yielded mutation frequencies as great as 50% that witnessed for the mutation frequency induced by the potent carcinogen far ultraviolet light. NUV alone was nontoxic and nonmutagenic at the doses of radiation used. Exposure of CHO cells in the dark to nontoxic dilutions of the process waters resulted in small but significant increases in 6-thioguanine resistent mutants (1-2 times background rates). The biological consequences resulting from the disposal of retort process waters into the delcate environment present in this oil shale region could be further complicated by this photoactivating process.
- Research Organization:
- Los Alamos National Lab., NM
- OSTI ID:
- 5305916
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cytotoxic and mutagenic properties of shale oil byproducts. II. Comparison of mutagenic effects at five genetic markers induced by retort process water plus near ultraviolet light in Chinese hamster ovary cells
Quantitative mammalian cell genetic toxicology: study of the cytotoxicity and mutagenicity of 70 individual environmental agents related to energy technologies and 3 subfractions of a crude synthetic oil in the CHO/HGPRT system. [Hamsters]
Related Subjects
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
SHALE OIL
BIOLOGICAL EFFECTS
CELL CULTURES
CELL KILLING
HAMSTERS
MUTAGENESIS
NEAR ULTRAVIOLET RADIATION
OVARIES
WASTE WATER
ANIMALS
BODY
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
FEMALE GENITALS
FOSSIL FUELS
FUELS
GONADS
HYDROGEN COMPOUNDS
LIQUID WASTES
MAMMALS
MINERAL OILS
OILS
ORGANIC COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RODENTS
ULTRAVIOLET RADIATION
VERTEBRATES
WASTES
WATER
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
560121 - Radiation Effects on Cells- External Source- (-1987)