Uptake and metabolism of retinol in cultured sertoli cells: evidence for a kinetic model
When cultured Sertoli cells derived from 20-day-old weanling rats were supplied (/sup 3/H) retinol bound to serum retinol binding protein-transthyretin complex, (/sup 3/H) retinol was rapidly incorporated and (/sup 3/H) retinyl esters were synthesized. Within 28 h after administration, 83% of the labeled retinoids were accounted for as retinyl esters (64% as retinyl palmitate). Sertoli cells derived from vitamin A deficient rats and supplied (/sup 3/H) retinol in culture under identical conditions likewise incorporated (/sup 3/H) retinol and synthesized retinyl esters. In contrast to normal Sertoli cells, vitamin A deficient Sertoli cells eventually metabolized virtually all of the cellular (/sup 3/H) retinol to retinyl esters. The primary metabolic fate of retinol administered to Sertoli cell cultures was the synthesis of retinyl esters under all conditions tested. However, administration of (/sup 3/H) retinol bound to serum retinol binding protein gave metabolic profiles having a higher proportion of retinyl esters and lower proportions of unresolved polar material than administration of (/sup 3/H) retinol alone. The kinetics of retinol uptake and intracellular retinyl ester synthesis in cultured Sertoli cells was complex. An initial, rapid phase of (/sup 3/H) retinol incorporation lasting 30 min was followed by a slower rate of incorporation and a concomitant decrease in the intracellular concentration of (/sup 3/H) retinol. During the time course the specific activity of (/sup 3/H) retinyl palmitate eventually exceeded that of intracellular (/sup 3/H) retinol. These observations suggest that two intracellular pools of retinol may exist in Sertoli cells.
- Research Organization:
- Washington State Univ., Pullman
- OSTI ID:
- 5303437
- Journal Information:
- Biochemistry; (United States), Vol. 26:23
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
RETINOIC ACID
BIOSYNTHESIS
TESTES
BIOCHEMICAL REACTION KINETICS
TRITIUM COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
RATS
SOMATIC CELLS
VITAMIN A
ANIMAL CELLS
ANIMALS
BODY
CARBOXYLIC ACID ESTERS
CHROMATOGRAPHY
ESTERS
GONADS
KINETICS
LABELLED COMPOUNDS
MALE GENITALS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
VITAMINS
550201* - Biochemistry- Tracer Techniques