Very-high-resolution two-dimensional gel electrophoresis of proteins using giant gels
Journal Article
·
· Anal. Biochem.; (United States)
An improved high-resolution two-dimensional gel system for separating complex protein mixtures is described that allows a threefold increase in the number of proteins detected. LIke the original 0'Farrell system, proteins are separated in the first dimension by isoelectric point and in the second dimension by size. The improved resolution results primarily from a 2.5-fold increase in the size of both dimensions. Although best resolution is obtained by application of <100 ..mu..g of protein containing >5 x 10/sup 6/ cpm, as much as 150 ..mu..g of protein may be applied without appreciable loss of resolution. Useful separations may be made with up to 1.5 mg. By doubling the thickness of both dimensions, as much as 3 mg of protein can be separated into 300 to 400 separate peaks.
- Research Organization:
- Univ. of Rochester School of Medicine and Dentistry, NY
- OSTI ID:
- 5302434
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 104:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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