Size and number of DNA molecules from Chinese hamster ovary cells determined by molecular autoradiography

Technical Report · Sun Jun 01 00:00:00 EDT 1980

DOI:https://doi.org/10.2172/5302185· OSTI ID:5302185

Todd, M. B.

A new method for visualization of separable subunits of DNA is described. Autoradiography of tritium-labeled DNA from one or a few nuclei, lysed with detergent, moderate salt, and proteases, and gently deposited on a filter, allows determination of subunit molecular weight, size distribution, number per nucleus, and organization. The shape of the size distribution of CHO subunit images is similar to that of CHO mitotic chromosomes, and the numbers of subunits per nucleus supports a model of eight subunits per chromosome.

• Research Organization: Texas Univ., Houston (USA)
• DOE Contract Number: AS05-76EV02832
• OSTI ID: 5302185
• Report Number(s): ORO-2832-212; TRN: 80-013929
• Resource Relation: Other Information: Thesis
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
MOLECULAR STRUCTURE
DNA
ANIMAL CELLS
AUTORADIOGRAPHY
CELL CULTURES
HAMSTERS
LABELLED COMPOUNDS
TRITIUM
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
RADIOISOTOPES
RODENTS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550301* - Cytology- Tracer Techniques