Aluminum fluoride induces phosphatidylinositol turnover, elevation of cytoplasmic free calcium, and phosphorylation of the T cell antigen receptor in murine T cells
Antigen activation of murine T lymphocytes leads to phosphorylation of three subunits of the murine T cell antigen receptor. Two kinases are activated in this process: protein kinase C which leads to phosphorylation of the ..gamma.. and, to a lesser extent, the epsilon subunits on serine residues and a tyrosine kinase which phosphorylates the p21 subunit. The authors sought to determine whether treatment of these cells with NaF could simulate any of these antigen-induced events. Indeed NaF treatment resulted in breakdown of polyphosphoinositides and production of phosphoinositols. This treatment also resulted in a rise in cytosolic free Ca/sup 2 +/. EGTA failed to block this rise suggesting that NaF liberated intracellular stores of Ca/sup 2 +/. Finally NaF treatment resulted in phosphorylation of the ..gamma.. and epsilon chains of the T cell receptor indistinguishable from the effects of phorbol esters. The NaF effect was potentiated by addition of AlCl/sub 3/ consistent with the view that the active moiety is AlF/sub 4//sup -/. The AlF/sub 4//sup -/-induced phosphorylations were abolished in cells in which protein kinase C was depleted by prior treatment with phorbol myristate acetate. All of these observations are compatible with the interpretation that the AlF/sub 4//sup -/ phosphorylation is mediated by protein kinase C. Antigen and anti-receptor antibody-induced receptor serine phosphorylation and phophatidylinositol turnover are blocked by raising intracellular levels of cyclic adenosin monophosphate. In contrast, AlF/sub 4//sup -/-induced effects were in sensitive to cyclic adenosine monmonophosphate
- Research Organization:
- National Institutes of Health, Bethesda, MD
- OSTI ID:
- 5301519
- Journal Information:
- J. Immunol.; (United States), Vol. 139:10
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ALUMINIUM FLUORIDES
BIOLOGICAL EFFECTS
PHORBOL ESTERS
RECEPTORS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
ANTIGENS
CALCIUM COMPOUNDS
CATIONS
ELECTROPHORESIS
ENZYME INDUCTION
INOSITOLS
IODINE 125
LYMPHOCYTES
MICE
PHOSPHORUS 32
PHOSPHORYLATION
PHOSPHOTRANSFERASES
TRITIUM COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
ALUMINIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CHARGED PARTICLES
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERS
FLUORIDES
FLUORINE COMPOUNDS
GENE REGULATION
HALIDES
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
IONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SACCHARIDES
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics