Estimation of the frequency of malformed sperm by slit scan flow cytometry
An investigation was made of the utility of Slit Scan Flow Cytometry (SSFCM) for measuring the frequencies of malformed sperm heads in control and mutagen treated B6C3F1/CRL mice. In SSFCM, fluorescence profiles of sperm heads stained with the DNA-specific fluorescent dye acriflavine were recorded for sperm flowing lengthwise through a 2.5-..mu..m-thick laser beam. Malformed sperm were detected as having fluorescence profiles that differed substantially from an average fluorescence profile for sperm from untreated mice. Fluorescence profiles were measured for 500 sperm per mouse from five control mice, five mice injected intraperitoneally daily for 5 days with a total of 375 mg/kg of body weight methyl methane sulfonate (MMS), and for 30 mice injected intraperitoneally daily for 5 days with total doses of procarbazine ranging from 125 mg/kg to 1250 mg/kg. Sperm were collected from the caudae epididymides 35 days after the last injection. Frequencies of malformed sperm in these samples were also estimated by visual analysis. All samples were analyzed in double blind fashion. The visual and SSFCM malformed sperm frequencies for the samples from control, MMS-treated, and procarbazine-treated mice were correlated. A dose effect was seen with both the visual and SSFCM estimates for the sperm from the procarbazine-treated mice. 8 references 3 figures.
- Research Organization:
- Univ. of California, Livermore, CA (United States)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5300431
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
METHYL METHANESULFONATE
BIOLOGICAL EFFECTS
SPERMATOZOA
MORPHOLOGY
ACRIFLAVINE
CELL FLOW SYSTEMS
COMPARATIVE EVALUATIONS
DNA
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
MEASURING METHODS
MICE
MUTAGENS
MUTATION FREQUENCY
ACRIDINES
AMINES
ANIMALS
AROMATICS
AZAARENES
AZINES
DATA
ESTERS
FLAVINES
GAMETES
GERM CELLS
HETEROCYCLIC COMPOUNDS
INFORMATION
MAMMALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRIDINES
RODENTS
SULFONIC ACID ESTERS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)