Adenosine (ADO) prevents phorbol myristate acetate (PMA) injury in rabbit lungs: Role of leukotrienes (LT)

Conference · Mon Mar 11 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5300123

Zanaboni, P; Bradley, J; Webster, R; Baudendistel, L; Dahms, T ^[1]

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1. St. Louis Univ., MO (United States)

Pretreatment with ADO prevents the PMA-induced increase in the fluid filtration coefficient (K{sub f}) in isolated perfused rabbit lungs. ADO can block the synthesis of LTs. This study determined whether the protective effect of ADO in PMA induced injury was due to decreased LT production. Isolated rabbit lungs were perfused with a 1:1 mixture of blood and 6% albumin in Krebs-Henseleit buffer. K{sub f} was measured after hemodynamic and weight stabilization and then 30 min after PMA. Perfusate samples were collected after each K{sub f} measurement for determination of LTC{sub 4} and LTD{sub 4} by RIA. The specific role of LT was studied by pretreating with MK-886, a LT synthesis inhibitor. In the vehicle pretreatment group, PMA caused a significant increase in K{sub f} and LTC{sub 4} + LTD{sub 4}. ADO inhibited both the increase in K{sub f} and in LTC{sub 4} + LTD{sub 4}. Pretreatment with MK-886 blocked the PMA-induced increase in LT but did not inhibit the K{sub f} increase. ADO alters the production of LTC{sub 4} + LTD{sub 4} following PMA; but it is probably not the mechanism by which ADO prevents the increase in microvascular permeability.

• OSTI ID: 5300123
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENOSINE
BIOLOGICAL EFFECTS
PHORBOL ESTERS
TOXICITY
PROSTAGLANDINS
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
LUNGS
PERFUSED ORGANS
RABBITS
RADIOIMMUNOASSAY
ANIMALS
BIOASSAY
BODY
CARCINOGENS
DIAGNOSTIC TECHNIQUES
ESTERS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RESPIRATORY SYSTEM
RIBOSIDES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology