Yeast redoxyendonuclease, a DNA repair enzyme similar to Escherichia coli endonuclease III
A DNA repair endonuclease (redoxyendonuclease) was isolated from bakers' yeast (Saccharomyces cerevisiae). The enzyme has been purified by a series of column chromatography steps and cleaves OsO/sub 4/-damaged, double-stranded DNA at sites of thymine glycol and heavily UV-irradiated DNA at sites of cytosine, thymine, and guanine photoproducts. The base specificity and mechanism of phosphodiester bond cleavage for the yeast redoxyendonuclease appear to be identical with those of Escherichia coli endonuclease III when thymine glycol containing, end-labeled DNA fragments of defined sequence are employed as substrates. Yeast redoxyendonuclease has an apparent molecular size of 38,000-42,000 daltons and is active in the absence of divalent metal cations. The identification of such an enzyme in yeast may be of value in the elucidation of the biochemical basis for radiation sensitivity in certain yeast mutants.
- Research Organization:
- Emory Univ. School of Medicine, Atlanta, GA (USA)
- OSTI ID:
- 5289022
- Journal Information:
- Biochemistry; (United States), Vol. 27:7
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
DNA
AUTORADIOGRAPHY
NUCLEASES
BIOCHEMISTRY
STRAND BREAKS
RADIOINDUCTION
DNA REPAIR
DNA SEQUENCING
ELECTROPHORESIS
ESCHERICHIA COLI
EXTREME ULTRAVIOLET RADIATION
OSMIUM OXIDES
PHOSPHORUS 32
SACCHAROMYCES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHALCOGENIDES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
FUNGI
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OSMIUM COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS ISOTOPES
PLANTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REFRACTORY METAL COMPOUNDS
REPAIR
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENT COMPOUNDS
ULTRAVIOLET RADIATION
YEASTS
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