Isolation, structure, and characterization of the RAD3 gene of the yeast
In Saccharomyces cerevisiae excision of UV-induced pyrimidine dimers from the DNA involves at least 10 genes. In this work, the RAD3 gene has been cloned, its nucleotide sequence determined, and some structural and functional features examined. The RAD3 gene codes for an mRNA of 2.5 kb. The RAD3 open reading frame is 2334 nucleotides long with an encoded protein of 89,779 daltons. Genomic deletions of the RAD3 gene are recessive lethal, demonstrating that it is an essential gene and suggesting that the RAD3 gene product plays a vital role in the cell in addition to its role in excision repair. To date, the RAD3 gene is the only RAD gene known to be essential for viability. It is known that the RAD1, RAD2, RAD4, RAD6, RAD7, RAD9, RAD10, RAD18, and RAD23 genes are not. A series of RAD3-lacZ fusions have been made across the N-terminal region of the RAD3 coding sequences and the subcellular localization of the fusion proteins determined. There exists an amino acid sequence within the first 32 RAD3 amino acids that allows for association of the RAD3-lacZ gene fusion with the nucleus. This region of the RAD3 protein shows no obvious similarity to any previously defined nuclear localization signal.
- Research Organization:
- Rochester Univ., NY (USA)
- OSTI ID:
- 5288425
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENES
CHEMICAL COMPOSITION
MOLECULAR STRUCTURE
PYRIMIDINE DIMERS
RADIOINDUCTION
SACCHAROMYCES CEREVISIAE
GENETIC RADIATION EFFECTS
AMINO ACID SEQUENCE
DNA
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
FUNGI
GENETIC EFFECTS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
SACCHAROMYCES
YEASTS
560130* - Radiation Effects on Microorganisms