Changes in numbers and types of mast cell colony-forming cells in the peritoneal cavity of mice after injection of distilled water: evidence that mast cells suppress differentiation of bone marrow-derived precursors
Two different types of cells in the peritoneal cavity of mice produce mast cell colonies in methylcellulose. Large mast cell colonies are produced by bone marrow-derived precursors resembling lymphoid cells by light microscopy (L-CFU-Mast), whereas medium and small mast cell colonies are produced by morphologically identifiable mast cells (M-CFU-Mast and S-CFU-Mast, respectively). In the present study we eradicated peritoneal mast cells by intraperitoneal (IP) injection of distilled water. The regeneration process was investigated to clarify the relationship between L-CFU-Mast, M-CFU-Mast, and S-CFU-Mast. After injection of distilled water, M-CFU-Mast and S-CFU-Mast disappeared, but L-CFU-Mast increased, and then M-CFU-Mast and S-CFU-Mast appeared, suggesting the presence of a hierarchic relationship. When purified peritoneal mast cells were injected two days after the water injection, the L-CFU-Mast did not increase. In the peritoneal cavity of WBB6F1-+/+ mice that had been lethally irradiated and rescued by bone marrow cells of C57BL/6-bgJ/bgJ (beige, Chediak-Higashi syndrome) mice, L-CFU-Mast were of bgJ/bgJ type, but M-CFU-Mast and S-CFU-Mast were of +/+ type. The injection of distilled water to the radiation chimeras resulted in the development of bgJ/bgJ-type M-CFU-Mast and then S-CFU-Mast. The presence of mast cells appeared to suppress the recruitment of L-CFU-Mast from the bloodstream and to inhibit the differentiation of L-CFU-Mast to M-CFU-Mast.
- Research Organization:
- Osaka Univ. Medical School (Japan)
- OSTI ID:
- 5287543
- Journal Information:
- Blood; (United States), Vol. 71:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BONE MARROW CELLS
CELL DIFFERENTIATION
MAST CELLS
COLONY FORMATION
CELL DIVISION
INTRAPERITONEAL INJECTION
MICE
PERITONEUM
PRECURSOR
RADIATION CHIMERAS
STEM CELLS
WATER
ANIMAL CELLS
ANIMALS
CHIMERAS
CONNECTIVE TISSUE CELLS
HYDROGEN COMPOUNDS
INJECTION
INTAKE
MAMMALS
MEMBRANES
MOSAICISM
OXYGEN COMPOUNDS
RODENTS
SEROUS MEMBRANES
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals