Enhanced capacity of DNA repair in human cytomegalovirus-infected cells
Plaque formation in Vero cells by UV-irradiated herpes simplex virus was enhanced by infection with human cytomegalovirus (HCMV), UV irradiation, or treatment with methylmethanesulfonate. Preinfection of Vero cells with HCMV enhanced reactivation of UV-irradiated herpes simplex virus more significantly than did treatment with UV or methylmethanesulfonate alone. A similar enhancement by HCMV was observed in human embryonic fibroblasts, but not in xeroderma pigmentosum (XP12BE) cells. It was also found that HCMV infection enhanced hydroxyurea-resistant DNA synthesis induced by UV light or methylmethanesulfonate. Alkaline sucrose gradient sedimentation analysis revealed an enhanced rate of synthesis of all size classes of DNA in UV-irradiated HCMV-infected Vero cells. However, HCMV infection did not induce repairable lesions in cellular DNA and did not significantly inhibit host cell DNA synthesis, unlike UV or methylmethanesulfonate. These results indicate that HCMV enhanced DNA repair capacity in the host cells without producing detectable lesions in cellular DNA and without inhibiting DNA synthesis. This repair appeared to be error proof for UV-damaged herpes simplex virus DNA when tested with herpes simplex virus thymidine kinase-negative mutants.
- OSTI ID:
- 5285011
- Journal Information:
- J. Virol.; (United States), Vol. 38:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
BIOLOGICAL REPAIR
METHYL METHANESULFONATE
BIOLOGICAL EFFECTS
PLAQUE FORMATION
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CELL CULTURES
COMPARATIVE EVALUATIONS
ENZYME ACTIVITY
HERPES SIMPLEX
MUTANTS
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM
BIOLOGICAL RECOVERY
DISEASES
ELECTROMAGNETIC RADIATION
ESTERS
INFECTIOUS DISEASES
MUTAGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
SULFONIC ACID ESTERS
SYNTHESIS
VIRAL DISEASES
550200* - Biochemistry
550400 - Genetics
550700 - Microbiology
560121 - Radiation Effects on Cells- External Source- (-1987)