A rapid radioimmunoassay using /sup 125/I-labeled staphylococcal protein A for antibody to varicella-zoster virus

Journal Article · Fri May 01 00:00:00 EDT 1981 · J. Infect. Dis.; (United States)

DOI:https://doi.org/10.1093/infdis/143.5.693· OSTI ID:5284248

Richman, D D; Cleveland, P H; Oxman, M N; Zaia, J A

A sensitive radioimmunoassay for serum antibody to varicella-zoster virus is described; it uses 125I-labeled staphylococcal protein A and a specially designed immunofiltration apparatus. The assay accurately distinguishes between individuals who are susceptible and those who are immune to infection with varicella-zoster virus. In addition, it can detect passive antibody in recipients of varicella-zoster immune globulin. This radioimmunoassay also detects the heterologous antibody responses that occasionally occur in patients infected with herpes simplex virus, which also have been detected by other antibody assays. The particular advantages of this assay are the use of noninfectious reagents, the speed of execution (less than 3 hr), the requirement for only small quantities of serum (30 microliters), the objectivity of end-point determination, and the capability of screening large numbers of sera. Consequently, this radioimmunoassay is especially useful for the rapid identification of susceptible individuals, which is essential for the appropriate management of patients and hospital personnel after exposure to varicella.

• OSTI ID: 5284248
• Journal Information: J. Infect. Dis.; (United States), Vol. 143:5
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
RADIOIMMUNOASSAY
IODINE 125
ISOTOPE APPLICATIONS
ACCURACY
ANTIGEN-ANTIBODY REACTIONS
HERPES SIMPLEX
HERPES ZOSTER
IMMUNE SERUMS
PATIENTS
PROTEINS
STAPHYLOCOCCUS
TIME DEPENDENCE
VIRUSES
BACTERIA
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MICROORGANISMS
NERVOUS SYSTEM DISEASES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
RADIOASSAY
RADIOISOTOPES
SKIN DISEASES
TRACER TECHNIQUES
VIRAL DISEASES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550701 - Microbiology- Tracer Techniques
550901 - Pathology- Tracer Techniques