Preparation of steroid radioligands for ultrafiltration assays by a solid-phase transport globulin method using concanavalin A-Sepharose

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Steroids; (United States)

DOI:https://doi.org/10.1016/0039-128X(85)90066-2· OSTI ID:5278426

Hiramatsu, R; Nisula, B C

Concanavalin A-Sepharose (Con A-Sepharose) was applied to separate non-protein-bound and albumin-bound radioactive impurities from steroid radioligands. Con A-Sepharose gel, plasma, and steroid radioligand were mixed, incubated, and then washed with buffer. This method was compared with an affinity diafiltration method which separates non-protein-bound radioactive impurities with a filter membrane. /sup 3/H-Water and /sup 3/H-estrone sulfate, chosen to serve as molecules representative of non-protein-bound and albumin-bound impurities, were removed quite effectively by the Con A-Sepharose method, while 85% of /sup 3/H-estrone sulfate could not be removed by the diafiltration method. Plasma unbound cortisol (F) and testosterone (T) values determined by ultrafiltration using /sup 3/H-F and /sup 3/H-T prepared by the Con A-Sepharose method were significantly lower than those determined using the radioligands unprocessed or prepared by the diafiltration method. The whole procedure of the Con A-Sepharose method takes only 3-4 hours. This method is a simple, rapid, and effective technique for preparation of steroid radioligands for plasma protein binding studies.

• Research Organization: National Institute of Child Health and Human Development, Bethesda, MD
• OSTI ID: 5278426
• Journal Information: Steroids; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
62 RADIOLOGY AND NUCLEAR MEDICINE
RADIOPHARMACEUTICALS
CHEMICAL PREPARATION
HYDROCORTISONE
IMPURITIES
LIGANDS
REMOVAL
STEROIDS
TESTOSTERONE
TRITIUM COMPOUNDS
ULTRAFILTRATION
ADRENAL HORMONES
ANDROGENS
ANDROSTANES
CORTICOSTEROIDS
DRUGS
FILTRATION
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PREGNANES
SEPARATION PROCESSES
STEROID HORMONES
SYNTHESIS
400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials
550601 - Medicine- Unsealed Radionuclides in Diagnostics