Effect of allicin (diallyl disulfide-oxide) on prostaglandin endoperoxide H/sub 2/ (PGH/sub 2/) and arachidonic acid (AA) metabolism and platelet aggregation
The authors report here the effects of pure allicin (the antibacterial component of GO), synthesized from diallyl disulfide and hydrogen peroxide, on human platelet aggregation, PGH/sub 2/ metabolism in microsomes of bovine lung (BL) and bovine coronary artery (BCA), homogenates of human platelet (HP), and on AA metabolism in HP. Allicin at 16 ..mu..g/ml to 160 ..mu..g/ml produced concentration-dependent inhibition of platelet aggregation to 1.6 mM AA and 2.8 ..mu..M U 46619, a stable analog of PGH/sub 2/ and a TXA/sub 2/ minic. BL (200 ..mu..g protein), BCA (10 ..mu..g protein), and HP (1500 ..mu..g protein) were incubated with 10 ..mu..M (/sup 14/C) PGH/sub 2/ +/- allicin. HP (1500 ..mu..g protein) were incubated with 20 ..mu..M (/sup 14/C) AA +/- allicin. Products were separated by TLC and quantified by radiochromatographic scan. Allicin in the concentration range of 10-/sup 6/M-10-/sup 3/M induced no change in the formation of prostacyclin by BL and BCA or of TXA/sub 2/ by BL and HP. These data suggest that the platelet antiaggregatory action of allicin is not due to inhibition of cyclooxygenase or TXA/sub 2/ synthetase in the human platelet, but may be related to interactions at the TXA/sub 2/ receptor or on cyclic nucleotide levels.
- Research Organization:
- Tulane Medical School, New Orleans, LA
- OSTI ID:
- 5277795
- Report Number(s):
- CONF-8604222-; TRN: 86-030487
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ARACHIDONIC ACID
METABOLISM
BLOOD COAGULATION
INHIBITION
DISULFIDES
BIOLOGICAL EFFECTS
PROSTAGLANDINS
BLOOD PLATELETS
CARBON 14 COMPOUNDS
CATTLE
CORONARIES
DOSE-RESPONSE RELATIONSHIPS
HOMOGENATES
LUNGS
MICROSOMES
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
ANIMALS
ARTERIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD VESSELS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CHROMATOGRAPHY
DOMESTIC ANIMALS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
ORGANS
RESPIRATORY SYSTEM
RUMINANTS
SEPARATION PROCESSES
VERTEBRATES
550501* - Metabolism- Tracer Techniques