Effects of ultraviolet radiation on dendritic cell viability and function

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5276592

Everson, M P; Spalding, D M; Koopman, W J

Ultraviolet radiation (UVR) has been used by several investigators to successfully abrogate accessory cell function, however its mode of action remains unclear. The findings indicate that dendritic cells (DC) are exquisitely sensitive to UVR and therefore, UVR may provide a good probe to study DC accessory cell function. The authors compared the in vitro effects of UVB and UVC on murine splenic DC viability over a dose range of 0-864 J/m/sup 2/. DC viability as a function of time was not significantly different from control for 108, 432, and 864 J/m/sup 2/ UVB at 0-4 hr post-treatment; however, DC viability decreased significantly in a UVR dose-dependent fashion for the 6-10 h post-treatment period. Fourfold less UVC dosages caused equivalent changes in DC viability indicating that higher frequency radiation is substantially more detrimental than UVB. The authors next compared the effect of UVR on viability to its effect on DC function in oxidative mitogenesis assays. UV exposure induced disproportionate decreases in DC function compared to viability (using 216 J/m/sup 2/ UVB, the viability as a percent of control was 90% and 43% at 10 and 20 h, respectively, while DC function was only 18% of the non-UVR treated control value). Thus, function appears to be more sensitive to UVR than viability and the authors have not been able to restore function with IL-1. Thus, DC are exquisitely sensitive to UVR and better understanding of the defects induced by UVR should increase the knowledge of how DC function in induction of lymphocyte responses.

• Research Organization: Univ. ov Alabama, Birmingham
• OSTI ID: 5276592
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

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