Reduced spleen natural killer cell activity in virally challenged iron-deficient rat pups
Neonatal iron deficiency has been shown to alter several aspects of immunity in rats. This study determined the effects of iron deficiency on cytotoxicity of virally-induced natural killer cells (NK) in spleen. Rats (n = 8-12/grp) were fed 6 (severe deficiency, SID), 10 (moderate deficiency, MID), or 250 (adequate iron status, AIS) ppm iron d 1 pregnancy through d 21 lactation. Litters were adjusted to 7 on d 2. On d 17 pups were challenged intraperitoneally with 5 x 10/sup 5/ plaque forming units of vaccinia virus. Spleens were collected 4 d later and cell suspensions prepared and pooled within each litter. After isolation of mononuclear cells on a Ficoll-Hypaque gradient, macrophages were removed, and the resulting lymphocytes were incubated with Cr/sup 51/ labelled Yac-1 target cells at effector:target ratios (E:T) of 10:1 and 50:1. Cytotoxicity of NK cells were measured after 4 and 16 hrs by the Cr/sup 51/ release assay. In SID and MID groups body weights, spleen weights, and hemoglobin levels were significantly lower than in AIS pups (p < 0.001). Spleen NK cell cytotoxicity was significantly impaired in SID and MID pups. Depending on the E:T and incubation time, SID and MID cells had activities 30-50% of AIS cells (p < 0.001). Both severe and moderate iron deficiency markedly impair the cytotoxic activity of spleen natural killer cells in suckling rats.
- Research Organization:
- Univ. of Illinois, Urbana
- OSTI ID:
- 5275153
- Report Number(s):
- CONF-8604222-; TRN: 86-030570
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
IRON
NUTRITIONAL DEFICIENCY
SPLEEN
CELL KILLING
CHROMIUM 51
IMMUNITY
LACTATION
LYMPHOCYTES
MACROPHAGES
NEONATES
PREGNANCY
RATS
TOXICITY
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
METALS
NUCLEI
ORGANS
PHAGOCYTES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TRANSITION ELEMENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
551001 - Physiological Systems- Tracer Techniques