Assembly of the yeast mitochondrial H/sup +/ATPase: regulation by the overproduction and availability of the nuclear encoded (F/sub 1/) subunits

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5272198

Burns, D; Lewin, A

The assembly of the mitochondrial ATPase was studied in vitro by incubating isolated yeast mitochondria with radiolabeled mitochondrial precursors and in vivo by pulse-labeling of intact yeast cells and spheroplasts. Newly assembled F/sub 1/ ATPase (radiolabeled) was assayed by immunoprecipitation using subunit-specific antisera directed to the ..cap alpha.. subunit of the complex. Using two different experimental approaches, the authors have provided evidence suggesting that isolated mitochondria possess pools of unassembled F/sub 1/..cap alpha.. subunits and possibly F/sub 1/..beta.. subunits. In addition, the kinetics of import suggest that the F/sub 1/..beta.. subunit was imported and assembled at a slower rate than either the F/sub 1/..cap alpha.. and F/sub 1/..gamma.. subunits. Thus, the appearance of the new ATPase could be limited by the availability of the ..beta.. subunits.

• Research Organization: Indiana Univ., Bloomington
• OSTI ID: 5272198
• Report Number(s): CONF-8606151-; TRN: 86-031454
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATP-ASE
MOLECULAR STRUCTURE
RADIOIMMUNOASSAY
BIOCHEMICAL REACTION KINETICS
IN VITRO
IN VIVO
LABELLED COMPOUNDS
MITOCHONDRIA
PRECURSOR
RADIOISOTOPES
YEASTS
ACID ANHYDRASES
CELL CONSTITUENTS
ENZYMES
FUNGI
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MICROORGANISMS
ORGANOIDS
PHOSPHOHYDROLASES
PLANTS
RADIOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques