Effect of urokinase on the proliferation of primary cultures of human prostatic cells

Journal Article · Sun Nov 01 00:00:00 EST 1987 · Thromb. Res.; (United States)

DOI:https://doi.org/10.1016/0049-3848(87)90441-5· OSTI ID:5272116

Kirchheimer, J C; Wojta, J; Hienert, G; Christ, G; Heger, M E; Pflueger, H B; Binder, B R

The effects of exogenously added urokinase type plasminogen activator, tissue type plasminogen activator, plasmin and thrombin on the proliferation of primary cultures of cells derived from prostatic hyperplasia or prostatic carcinomas were investigated by measuring the incorporation of /sup 3/H-thymidine into the cultures. Addition of urokinase type plasminogen activator (1.35 x 10(-9) M) or thrombin (10(-7) M) to the culture medium caused a two-fold increase of /sup 3/H-thymidine incorporation, regardless of the origin of the prostatic cells. Tissue type plasminogen activator did not alter the rate of /sup 3/H-thymidine incorporation, whereas plasmin caused a 25% decrease of /sup 3/H-thymidine incorporation in all cultures.

• Research Organization: Univ. of Vienna (Austria)
• OSTI ID: 5272116
• Journal Information: Thromb. Res.; (United States), Vol. 48:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROSTATE
NEOPLASMS
TUMOR CELLS
CELL PROLIFERATION
UROKINASE
BIOCHEMICAL REACTION KINETICS
DNA REPLICATION
FIBRINOLYSIN
THROMBIN
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
AZINES
BODY
COAGULANTS
DISEASES
DRUGS
ENZYMES
FIBRINOLYTIC AGENTS
GLANDS
HEMATOLOGIC AGENTS
HEMOSTATICS
HETEROCYCLIC COMPOUNDS
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MALE GENITALS
NONSPECIFIC PEPTIDASES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
SERINE PROTEINASES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques