In vitro binding of steroid hormones by natural and purified fibers
The in vitro binding of estrone, estradiol-17..beta.., estriol, testosterone, dihydrotestosterone, and estrone-3-glucuronide by wheat, oat and corn brans, oat hulls, cellulose, lignin, and cholestyramine resin was measured. Steroid binding was carried out by mixing 50 mg of binding substance with varying substrate quantities (0.037 ..mu..Ci; 0.50-2.51 pmol/incubation) of /sup 3/H-estrone, /sup 3/H-estradiol-17..beta.., /sup 3/H-estriol, /sup 3/H-estrone-3-glucuronide, /sup 4/H-testosterone, and /sup 370/C for 1 hr with shaking. Following centrifugation of the reaction mixture, a 1 ml aliquot was analyzed for radioactivity. The extent of steroid sequestration was characteristic and reproducible for each hormone. Cholestyramine bound an average of 90% of all the steroids tested, whereas cellulose bound the least (12%). Of the other substances tested, lignin bound 87%; wheat and oat grans, 45% each; corn bran, 44%; and oat hulls, 32% of the unconjugated hormones. The conjugated steroid was less likely to bind than the unconjugated steroids. Lignin appeared to be an important component in the interaction with steroid hormones. The results support the hydrophobic of nature of adsorption and suggest that the components of the fiber in diet should be considered separately when evaluating in vivo metabolic effects. Implications include the possible modification of hormone-dependent cancer risk through dietary intervention.
- Research Organization:
- Loma Linda Univ., CA
- OSTI ID:
- 5271367
- Report Number(s):
- CONF-8604222-; TRN: 86-031484
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ESTRADIOL
BIOCHEMICAL REACTION KINETICS
ESTRIOL
ESTRONE
TESTOSTERONE
CELLULOSE
CENTRIFUGATION
DIET
GLUCURONIDE CONJUGATES
IN VITRO
LIGNIN
METABOLISM
OATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
WHEAT
ANDROGENS
ANDROSTANES
CARBOHYDRATES
CEREALS
ESTRANES
ESTROGENS
GRASS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
METABOLITES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
550501* - Metabolism- Tracer Techniques
550600 - Medicine