Use of a plasmid DNA probe to monitor populations of Bacillus pumilus inoculant strains in hay
- Pioneer Hi-Bred International Inc., Johnston, IA (USA)
The authors are evaluating naturally occurring isolates of Bacillus pumilus for use as microbial hay preservatives. Seven isolates of B, pumilus from hay contained a 42-kb cryptic plasmid (pMGD296). They wished to determine whether pMGD296 could be used as a molecular marker to follow populations of these isolates in hay over time. Southern blots and colony blots of 69 isolates of B. pumilus and other Bacillus spp. were probed with {sup 32}P-labeled pMGD296. Twenty-nine probe-positive isolates were identified; of these, 28 contained a plasmid with a restriction profile identical to that of pMGD296. One isolate from untreated hay contained a 40-kb plasmid (pMGD150) that was homologous to pMGD296 but had a different restriction fragment pattern. Regions of homology between the two plasmids were identified by Southern blotting, and a 1.9-kb HindIII-PstI fragment of pMGD296 lacking strong homology to pMGD150 was cloned in pUC18. The cloned fragment hybridized only with isolates containing pMGD296 and was used to estimate populations of these isolates in treated and untreated hay.
- OSTI ID:
- 5271353
- Journal Information:
- Applied and Environmental Microbiology; (United States), Vol. 57:3; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOASSAY
BACILLUS
BIOLOGICAL MARKERS
DNA-CLONING
PHOSPHORUS 32
PLASMIDS
TRACER TECHNIQUES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CLONING
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
HYBRIDIZATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
553003* - Agriculture & Food Technology- Pest & Disease Control- (1987-)