Semiquantitative probe for radiation-induced normal tissue damage at the molecular level
Sheep antibodies to bovine type I collagen were employed in the immunohistochemical detection of type I collagen in lung tissue sections of irradiated LAF1 mice. A video image digitizing system was developed to estimate collagen levels, by assigning a numerical value (0-63) to each of approximately 53,800 picture elements (pixels) in the microscope field, according to the collagen-dependent fluorescence intensity at each locus. For lungs harvested 52 weeks subsequent to graded doses of 60Co gamma radiation between 0 and 10 Gy, a dose-dependent increase in type I collagen was observed in the alveolar walls. A reproducible increase was evident for doses as low as 5 Gy: doses of 7 to 10 Gy elicited type I collagen levels significantly elevated with respect to those of age-matched controls. These results are consistent with a role for type I collagen in the development of radiation-induced pulmonary fibrosis. The assay system developed here will be used to explore the role of connective tissue macromolecules in the development of radiation pneumonitis and fibrosis.
- Research Organization:
- Cross Cancer Institute, Edmonton, Alberta
- OSTI ID:
- 5268868
- Journal Information:
- Radiat. Res.; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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COLLAGEN
HISTOLOGICAL TECHNIQUES
LUNGS
BIOLOGICAL RADIATION EFFECTS
ANTIBODIES
COBALT 60
GAMMA RADIATION
MICE
RADIATION INJURIES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
COBALT ISOTOPES
ELECTROMAGNETIC RADIATION
INJURIES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SCLEROPROTEINS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals