Glucocorticoids inhibit coordinated translation of. cap alpha. - and. beta. -globin mRNAs in Friend erythroleukemia cells

Journal Article · Thu Dec 01 00:00:00 EST 1983 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(83)90483-6· OSTI ID:5267347

Papaconstantinou, J; Stewart, J A; Rabek, J P; McClintock, P R; Wong, E Y

The dimethylsulfoxide (Me/sub 2/SO)-mediated induction of hemoglobin synthesis in Friend erythroleukemia cells is inhibited by the glucocorticoids hydrocortisone, dexamethasone, and fluocinolone acetonide; hydrocortisone, at concentrations of 10/sup -5/ to 10/sup -8/ M inhibits by 90-30% and fluocinolone acetonide at concentrations of 10/sup -8/ to 10/sup -11/ M shows a greater than 90% inhibition. At these concentrations the hormones have no effect on cell growth or viability. In this study it has been shown that there is a group of proteins, including the ..cap alpha..- and ..beta..-globins, whose regulation is associated with the induction of Friend erythroleukemia cell differentiation, and that the expression of these, in addition to ..cap alpha..- and ..beta..-globin, is affected by glucocorticoids. It is concluded that, although the translation of ..cap alpha..- and ..beta..-globin mRNA is a major site of inhibition by glucocorticoids, there is a detectable amount of ..cap alpha..- and ..beta..-globin mRNA translation which results in unequal amounts of globin synthesis and an overall more potent inhibition of hemoglobin formation.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5267347
• Journal Information: Arch. Biochem. Biophys.; (United States), Vol. 227:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOCORTICOIDS
BIOLOGICAL EFFECTS
HEMOGLOBIN
BIOSYNTHESIS
MESSENGER-RNA
INHIBITION
CELL DIFFERENTIATION
DEXAMETHASONE
DMSO
GLOBULINS-ALPHA
GLOBULINS-BETA
HYDROCORTISONE
TUMOR CELLS
ADRENAL HORMONES
ANIMAL CELLS
CARBOXYLIC ACIDS
CORTICOSTEROIDS
GLOBIN
GLOBULINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
KETONES
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PIGMENTS
PORPHYRINS
PREGNANES
PROTEINS
RNA
STEROID HORMONES
STEROIDS
SULFOXIDES
SYNTHESIS
550200* - Biochemistry