Immunosuppressive mechanisms in protein-calorie malnutrition

Journal Article · Thu Aug 01 00:00:00 EDT 1991 · Surgery; (United States)

OSTI ID:5267307

Redmond, H P; Shou, J; Kelly, C J; Schreiber, S; Miller, E; Leon, P; Daly, J M ^[1]

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1. Univ. of Pennsylvania School of Medicine, Philadelphia (USA)

Protein-calorie malnutrition (PCM) induces immunosuppression leading to increased mortality rates. Impaired macrophage respiratory burst activity (superoxide anion (O2-) generation) occurs in PCM, but cellular mechanisms are unclear. The major pathway resulting in O2- production involves inositol lipid-dependent signal transduction. This study examined the effect of mild versus severe PCM on macrophage O2- generating signal transduction pathways specific for responses to Candida albicans. Mice (CFW/Swiss Webster: n = 300) were randomized to either control or low protein diets for 3 or 8 weeks. Peritoneal macrophages were harvested for O2- production, mannose-fucose receptor (MFR) expression, membrane phospholipid analysis, arachidonic acid (AA) content, prostaglandin E2 (PGE2) production, and protein kinase C levels. O2- release was impaired in both mild and severe PCM. MFR expression was also decreased at these time points. Inositol lipid content was significantly lower at the 8-week time point only, although PGE2 and AA were significantly higher in the low protein diet group at 3 weeks. Protein kinase C levels were unchanged by PCM. Thus, mild PCM significantly increases macrophage-PGE2 production secondary to increased AA phospholipid content, with subsequent inhibition of O2- and MFR expression. Severe PCM inhibits macrophage (O2-) through depletion of critical membrane phospholipid components with subsequent impairment in signal transduction.

• OSTI ID: 5267307
• Journal Information: Surgery; (United States), Vol. 110:2; ISSN 0039-6060
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MACROPHAGES
BIOLOGICAL FUNCTIONS
PROTEINS
NUTRITIONAL DEFICIENCY
ARACHIDONIC ACID
BIOLOGICAL PATHWAYS
CANDIDA
DIET
IMMUNOSUPPRESSION
IODINE ISOTOPES
MEMBRANE PROTEINS
MICE
NUTRITION
PHOSPHOTRANSFERASES
PROSTAGLANDINS
SUPEROXIDE RADICALS
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
ENZYMES
EUMYCOTA
FUNGI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
RADICALS
RODENTS
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
YEASTS
550201* - Biochemistry- Tracer Techniques