Administration of pyrene lipids by receptor-mediated endocytosis and their degradation in skin fibroblasts

Journal Article · Tue Oct 01 00:00:00 EDT 1991 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(91)90244-O· OSTI ID:5267137

Agmon, V; Dinur, T; Cherbu, S; Dagan, A; Gatt, S ^[1]

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1. Hebrew Univ., Jerusalem (Israel)

Sphingomyelin and seven glycosphingolipids were labeled with the fluorescent probe pyrene and administered into cultured fibroblasts by receptor-mediated endocytosis. For this purpose pyrene sphingomyelin or mixtures of pyrene glycolipid and unlabeled sphingomyelin were dispersed as small, unilamellar liposomes. Apolipoprotein E was then added and the receptor for this ligand on the cell surface was utilized for uptake of the liposomes and their transport to the lysosomes, where the respective pyrene lipids were degraded. Following incubation with each of the respective pyrene lipids, only the administered compound and the pyrene ceramide were present; intermediate hydrolysis products were not detected. This indicated that, in skin fibroblasts, the lysosomal ceramidase was limiting and controlled the rate of total degradation of the pyrene sphingolipids.

• OSTI ID: 5267137
• Journal Information: Experimental Cell Research; (United States), Vol. 196:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GLYCOLIPIDS
BIODEGRADATION
PYRENE
BIOLOGICAL EFFECTS
SPHINGOMYELINS
APOLIPOPROTEINS
CELL CULTURES
FIBROBLASTS
LIPIDS
RECEPTORS
SKIN
ANIMAL CELLS
AROMATICS
BODY
CARBOHYDRATES
CHEMICAL REACTIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DECOMPOSITION
ESTERS
HYDROCARBONS
LIPOPROTEINS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
PROTEINS
SACCHARIDES
SOMATIC CELLS
560300* - Chemicals Metabolism & Toxicology