Biochemical analysis of murine leukemia viruses isolated from radiation-induced leukemias of strain BALB/c

Journal Article · Fri Feb 01 00:00:00 EST 1980 · J. Virol.; (United States)

OSTI ID:5262283

Ellis, R W ^[1]; Hopkins, N; Fleissner, E

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1. Memorial Sloan-Kettering Cancer Center, New York, NY

Murine leukemia viruses isolated from radiation-induced BALB/c leukemias were characterized with respect to viral proteins and RNA. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the viral structural proteins revealed that for p12, p15, p30, and gp70, three to four electrophoretic variants of each could be detected. There was no correlation found between any of these mobilities and N- or B-tropism of the viruses. Proteins of all xenotropic viral isolates were identical in their gel electrophoretic profiles. The similar phenotypes of multiple viral clones from individual leukemias and of isolates grown in different cells suggest that the polymorphism of ecotropic viruses was generated in vivo rather than during in vitro virus growth. By two-dimensional fingerprinting of RNase T1-resistant oligonucleotides from 70S viral RNA, the previously reported association of N- and B-tropism with two distinct oligonucleotides was confirmed. The presence of two other oligonucleotides was correlated with positive and negative phenotypes of the virus-coded G/sub IX/ cell surface antigen. The RNAs of two B-tropic isolates with distinctive p15 and p12 phenotypes differed from the RNA of a prototype N-tropic virus by the absence of three oligonucleotides mapping in the 5' portion (gag region) of the prototype RNA. In addition, one small-plaque B-tropic virus displayed extensive changes in the RNA sequences associated with the env region of the prototype.

• OSTI ID: 5262283
• Journal Information: J. Virol.; (United States), Vol. 33:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
LEUKEMIA
RADIOINDUCTION
LEUKEMIA VIRUSES
BIOCHEMISTRY
PHENOTYPE
QUALITATIVE CHEMICAL ANALYSIS
ELECTROPHORESIS
MICE
NUCLEOTIDES
PROTEINS
RNA
RNA-ASE
ANIMALS
CHEMICAL ANALYSIS
CHEMISTRY
DISEASES
ENZYMES
HEMIC DISEASES
MAMMALS
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHOTRANSFERASES
RODENTS
TRANSFERASES
VERTEBRATES
VIRUSES
560152* - Radiation Effects on Animals- Animals
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