Spectroscopic studies of copper enzymes

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5253653

Dooley, D M; Moog, R; Zumft, W; Koenig, S H; Scott, R A; Cote, C E; McGuirl, M

Several spectroscopic methods, including absorption, circular dichroism (CD), magnetic CD (MCD), X-ray absorption, resonance Raman, EPR, NMR, and quasi-elastic light-scattering spectroscopy, have been used to probe the structures of copper-containing amine oxidases, nitrite reductase, and nitrous oxide reductase. The basic goals are to determine the copper site structure, electronic properties, and to generate structure-reactivity correlations. Collectively, the results on the amine oxidases permit a detailed model for the Cu(II) sites in these enzymes to be constructed that, in turn, rationalizes the ligand-binding chemistry. Resonance Raman spectra of the phenylhydrazine and 2,4-dinitrophenyl-hydrazine derivatives of bovine plasma amine oxidase and models for its organic cofactor, e.g. pyridoxal, methoxatin, are most consistent with methoxatin being the intrinsic cofactor. The structure of the Cu(I) forms of the amine oxidases have been investigated by X-ray absorption spectroscopy (XAS); the copper coordination geometry is significantly different in the oxidized and reduced forms. Some anomalous properties of the amine oxidases in solution are explicable in terms of their reversible aggregation, which the authors have characterized via light scattering. Nitrite and nitrous oxide reductases display several novel spectral properties. The data suggest that new types of copper sites are present.

• Research Organization: Amherst College, MA
• OSTI ID: 5253653
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
OXIDOREDUCTASES
MOLECULAR STRUCTURE
COPPER
ELECTRON SPIN RESONANCE
MAGNETIC CIRCULAR DICHROISM
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
QUASI-ELASTIC SCATTERING
RAMAN SPECTRA
STRUCTURE-ACTIVITY RELATIONSHIPS
X-RAY SPECTRA
DICHROISM
DIRECT REACTIONS
ELEMENTS
ENZYMES
MAGNETIC RESONANCE
METALS
NUCLEAR REACTIONS
QUASI-FREE REACTIONS
RESONANCE
SCATTERING
SPECTRA
TRANSITION ELEMENTS
550201* - Biochemistry- Tracer Techniques
550600 - Medicine