Bioconversion of N-octane to octanoic acid by a recombinant Escherichia coli cultured in a two-liquid phase bioreactor

Journal Article · Mon Apr 01 00:00:00 EST 1991 · Bio/Technology; (United States)

DOI:https://doi.org/10.1038/nbt0491-367· OSTI ID:5250723

Favre-Bulle, O; Schouten, T; Kingma, J; Witholt, B ^[1]

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1. Univ. of Groningen (Netherlands)

The alk genes from the catabolic OCT plasmid of Pseudomonas oleovorans, which encode the enzymes involved in the oxidation of n-alkanes to carboxylic acids, were introduced into E. coli W3110. The resulting recombinant converts n-octane in a two-liquid phase medium into the corresponding alkanoate and excretes this compound into the aqueous phase. The rate of octanoic acid production by the recombinant E. coli is equal to or better than the alkane oxidation rate of P. oleovorans, suggesting that two-liquid phase fermentations with E. coli might have future industrial applications.

• OSTI ID: 5250723
• Journal Information: Bio/Technology; (United States), Vol. 9:4; ISSN 0733-222X
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
OCTANE
BIODEGRADATION
OCTANOIC ACID
BIOSYNTHESIS
ESCHERICHIA COLI
GENES
GENETIC ENGINEERING
OXIDATION
PLASMIDS
PSEUDOMONAS
RECOMBINANT DNA
ALKANES
BACTERIA
BIOTECHNOLOGY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
DNA
HYDROCARBONS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
SYNTHESIS
090900* - Biomass Fuels- Processing- (1990-)