Effects of dexamethasone on the synthesis and secretion of galaptin by vascular smooth muscle cells

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5246786

Sanford, G L; Harris-Hooker, S A

The effects of dexamethasone (Dex) on the synthesis and secretion of beta-galactoside specific lectin (galaptin) was examined in cultured primate aortic smooth muscle cells (SMC). SMC cells were treated with 0.15 ..mu..M Dex during their proliferative phase to confluency, and after reaching confluency. Both cultures were labeled with (/sup 3/H)-phenylalanine (phe) for 24 h following exposure to Dex. Incorporation of phe into galaptin increased twofold in the medium from Dex treated confluent cultures, when serum was present. No change was found in incorporation when serum was removed prior to Dex treatment. Phe incorporation into total protein was also increased twofold by Dex treatment of SMC in the presence of serum, but there was a 1.4-fold increase when serum was absent. Dex did not affect the incorporation of phe into either total protein or galaptin in the cell layer of confluent cultures in the presence of serum, but caused a twofold increase in its absence. There was no effect of Dex on the incorporation of phe into galaptin or total protein in either the medium or cell layer of cultures given Dex during their proliferative phase. Dex retarded the growth of SMC, and lowered the total protein content of the cell layer. The results show that vascular smooth muscle cells synthesize and secrete galaptin and that Dex acts directly on confluent SMC to increase galaptin synthesis and secretion. Serum seems to modulate the effect of Dex.

• Research Organization: Morehouse School of Medicine, Atlanta, GA
• OSTI ID: 5246786
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CELL PROLIFERATION
DEXAMETHASONE
BIOLOGICAL EFFECTS
LECTINS
BIOSYNTHESIS
SECRETION
CELL CULTURES
MUSCLES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
CORTICOSTEROIDS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PREGNANES
STEROID HORMONES
STEROIDS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques