Endotoxin induction of an inhibitor of plasminogen activator in bovine pulmonary artery endothelial cells
The effects of bacterial lipopolysaccharide (endotoxin) on the fibrinolytic activity of bovine pulmonary artery endothelial cells were examined. Endotoxin suppressed the net fibrinolytic activity of cell extracts and conditioned media in a dose-dependent manner. The effects of endotoxin required at least 6 h for expression. Cell extracts and conditioned media contained a 44-kDa urokinase-like plasminogen activator. Media also contained multiple plasminogen activators with molecular masses of 65-75 and 80-100 kDa. Plasminogen activators in extracts and media were unchanged by treatment of cells with endotoxin. Diisopropyl fluorophosphate (DFP)-abolished fibrinolytic activity of extracts and conditioned media. DFP-treated samples from endotoxin-treated but not untreated cells inhibited urokinase and tissue plasminogen activator, but not plasmin. Inhibitory activity was lost by incubation at pH 3 or heating to 56/sup 0/C for 10 min. These treatments did not affect inhibitory activity of fetal bovine serum. Incubation of /sup 125/I-urokinase with DFP-treated medium from endotoxin-treated cells produced an inactive complex with an apparent molecular mass of 80-85 kDa.
- Research Organization:
- Miami Heart Institute, FL
- OSTI ID:
- 5246441
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 261:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
FIBRINOLYSIS
ENDOTOXINS
BIOCHEMICAL REACTION KINETICS
INHIBITION
CATTLE
DOSE-RESPONSE RELATIONSHIPS
ENDOTHELIUM
ENZYME INHIBITORS
EXPERIMENTAL DATA
IODINE 125
LABELLED COMPOUNDS
MOLECULAR WEIGHT
PH VALUE
PLASMINOGEN
TEMPERATURE EFFECTS
TRACER TECHNIQUES
UROKINASE
ANIMAL TISSUES
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BODY
CHEMICAL REACTIONS
DATA
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
HYDROLASES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MATERIALS
NONSPECIFIC PEPTIDASES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
PEPTIDE HYDROLASES
PROTEOLYSIS
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
TISSUES
TOXIC MATERIALS
TOXINS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques