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Title: Radioimmunoassay analysis of baculovirus granulins and polyhedrins

Abstract

Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera. Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica (Ac) polyhedrin and Trichoplusia ni (Tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (RIA). Ac polyhedrin and Tn granulin antisera recognized antigenic determinants on several baculovirus polyhedrin and granulin proteins even though the heterologous proteins had different immunoreactivities when compared by competition radioimmunoassay. Antigenic differences among granulin and polyhedrin proteins were also detected by altered slopes of the competition reaction curves. Antiserum raised against Ac polyhedrin which was purified in the presence of SDS was tested by competition RIA for its ability to detect and react with native polyhedrin produced in the infected TN-368 cells. Ac polyhedrin antiserum had similar if not identical ability to bind to native polyhedrin and to polyhedrin purified in the presence of SDS.

Authors:
;
Publication Date:
Research Org.:
Texas A and M Univ., College Station
OSTI Identifier:
5246232
Resource Type:
Journal Article
Journal Name:
Virology; (United States)
Additional Journal Information:
Journal Volume: 103:1
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; NUCLEOPROTEINS; RADIOIMMUNOASSAY; BIOCHEMICAL REACTION KINETICS; CHEMICAL PREPARATION; DIAGNOSIS; ENCAPSULATION; IMMUNE SERUMS; IODINE 125; VIRUSES; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MICROORGANISMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PARASITES; PROTEINS; RADIOASSAY; RADIOISOTOPES; REACTION KINETICS; SYNTHESIS; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 550701 - Microbiology- Tracer Techniques

Citation Formats

Summers, M D, and Hoops, P. Radioimmunoassay analysis of baculovirus granulins and polyhedrins. United States: N. p., 1980. Web. doi:10.1016/0042-6822(80)90128-2.
Summers, M D, & Hoops, P. Radioimmunoassay analysis of baculovirus granulins and polyhedrins. United States. https://doi.org/10.1016/0042-6822(80)90128-2
Summers, M D, and Hoops, P. 1980. "Radioimmunoassay analysis of baculovirus granulins and polyhedrins". United States. https://doi.org/10.1016/0042-6822(80)90128-2.
@article{osti_5246232,
title = {Radioimmunoassay analysis of baculovirus granulins and polyhedrins},
author = {Summers, M D and Hoops, P},
abstractNote = {Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera. Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica (Ac) polyhedrin and Trichoplusia ni (Tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (RIA). Ac polyhedrin and Tn granulin antisera recognized antigenic determinants on several baculovirus polyhedrin and granulin proteins even though the heterologous proteins had different immunoreactivities when compared by competition radioimmunoassay. Antigenic differences among granulin and polyhedrin proteins were also detected by altered slopes of the competition reaction curves. Antiserum raised against Ac polyhedrin which was purified in the presence of SDS was tested by competition RIA for its ability to detect and react with native polyhedrin produced in the infected TN-368 cells. Ac polyhedrin antiserum had similar if not identical ability to bind to native polyhedrin and to polyhedrin purified in the presence of SDS.},
doi = {10.1016/0042-6822(80)90128-2},
url = {https://www.osti.gov/biblio/5246232}, journal = {Virology; (United States)},
number = ,
volume = 103:1,
place = {United States},
year = {Thu May 01 00:00:00 EDT 1980},
month = {Thu May 01 00:00:00 EDT 1980}
}