Seed-protein genes and the regulation of their expression. Progress report
Research has focused primarily on the isolation and identification of mRNAs directing the synthesis of major protease inhibitors of soybean seeds, the so called Bowman-Birk inhibitor and the family of related iso-inhibitors designated PI I-IV. A major objective of this research was to develop methods for detecting the primary translation products of the mRNA in a cell-free translation system, and use this procedure to identify the corresponding sequences among cDNA clones of the mRNAs. These clones are eventually to be used to characterize the expression of these genes during soybean embryo development, and to isolate and characterize the genes encoding these proteins in soybean and related legumes. We are also examining the potential for using Xenopus oocytes as a system for measuring transcription of plant genes. Research progress is summarized.
- Research Organization:
- Purdue Univ., Lafayette, IN (USA). Dept. of Botany and Plant Pathology
- DOE Contract Number:
- AC02-80ER10715
- OSTI ID:
- 5245257
- Report Number(s):
- DOE/ER/10715-2; ON: DE82016815
- Country of Publication:
- United States
- Language:
- English
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