Studies on thyroglobulin-specific suppressor T cell function in autoimmune thyroid disease
T cell regulation of the generation of thyroglobulin plaque-forming cells (Tg PFC) and protein A plaque-forming cells (Prot A PFC) was investigated using lymphocytes from patients with autoimmune thyroid disease. T and B cell mixed cultures (T-B MC) were carried out without mitogenic or antigenic stimulation to identify physiological T cell effects in the system. Tg PFC were found in 8 (44%) of 18 patients who had high titers of thyroglobulin antibody in their sera. Tg-specific and nonspecific immunoregulation by T cells from patients and normal subjects was studied using B cells from these eight patients in the T-B MC system. Remarkably lower values of Tg PFC induction compared to Prot A PFC induction were found after T cell addition. Normal T cells inhibited Tg PFC induction, but patient T cells did not, while the same extent of helper effects were found on Prot A PFC induction by the addition of patient and normal T cells. Irradiation (1500 rads) of T cells from patients and normal subjects significantly enhanced both Tg PFC and Prot A PFC induction. Thus, Tg-specific suppressor T cells are present in all normal subjects as part of the radiosensitive suppressor T cell subset. The increase in Tg-PFC caused by irradiation-induced inhibition of Tg-specific suppressor T cell function was significantly greater in normal subjects than in patients. Histamine type 2 receptor-bearing T cells inhibited Prot A PFC induction, but not Tg PFC induction, in the autologous T-B MC system. No Tg PFC were induced from normal B cells in any combination with untreated T cells, irradiated T cells, or histamine type 2 receptor-negative T cells from patients or normal subjects.
- Research Organization:
- Univ. of Chicago, IL
- OSTI ID:
- 5244070
- Journal Information:
- J. Clin. Endocrinol. Metab.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LYMPHOCYTES
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL RADIATION EFFECTS
ANTIBODY FORMATION
GOITER
HISTAMINE
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PATIENTS
RADIATION DOSES
RADIOSENSITIVITY
RECEPTORS
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THYROIDITIS
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AZOLES
BIOLOGICAL EFFECTS
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CONNECTIVE TISSUE CELLS
DISEASES
DOSES
ENDOCRINE DISEASES
GLOBULINS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
LEUKOCYTES
MATERIALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
RADIATION EFFECTS
SOMATIC CELLS
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