Synthesis of peptide fragments of the hemagglutinin of influenza virus A/Aichi/2/68 (H/sub 3/N/sub 2/)
Abstract
Peptides corresponding to sections 122-133, 136-147, and 154-164 of the heavy chain of the hemagglutinin of influenza virus A/Aichi/2/68 (H/sub 3/N/sub 2/) have been synthesized. The synthesis was performed by the stepwise growth of the peptide chain by means of activated esters of Boc-amino acids and the condensation of peptide blocks by the carbodiimide method in solution. The colored 2-(4-(phenylazo)benylsulfonyl)ethyl group, which can be removed by bases, was used for the protection of the C-terminal carboxy groups of the peptides and peptide blocks, facilitating the purification of the peptides synthesized. After the elimination of the N- and C-terminal carboxy groups of the peptides and peptide blocks, facilitating the purification of the peptides synthesized. After the elimination of the N- and C-terminal protective groups, the peptides were deblocked with 1 M trifluoromethanesulfonic acid in trifluoroacetic acid containing 10% of thioanisole and were purified by prepatative reversed-phase chromatography. Conjugates of the peptides synthesized with bovine serum albumin have been obtained.
- Authors:
- Publication Date:
- Research Org.:
- All-Union Scientific-Research Institute of Molecular Biology, Novosibirsk (USSR)
- OSTI Identifier:
- 5244049
- Resource Type:
- Journal Article
- Journal Name:
- Sov. J. Bioorg. Chem. (Engl. Transl.); (United States)
- Additional Journal Information:
- Journal Volume: 11:8; Other Information: Translated from Bioorganicheskaya Khim.; 11: No. 8, 1037-1047(Aug 1985)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; HEMAGGLUTININS; AMINO ACID SEQUENCE; BIOLOGICAL EFFECTS; BIOSYNTHESIS; RADIOIMMUNOASSAY; PEPTIDES; ACETIC ACID; ACYLATION; AMINO ACIDS; ANTIGENS; CARBON 14 COMPOUNDS; CARBOXYLIC ACID ESTERS; DEHYDROCYCLIZATION; INFLUENZA VIRUSES; LABELLING; ORGANIC FLUORINE COMPOUNDS; SULFONIC ACID ESTERS; TRITIUM COMPOUNDS; VACCINES; AGGLUTININS; ANTIBODIES; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; ESTERS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MICROORGANISMS; MOLECULAR STRUCTURE; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PARASITES; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; SYNTHESIS; TRACER TECHNIQUES; VIRUSES; 550201* - Biochemistry- Tracer Techniques
Citation Formats
Samukov, V V, Kalashnikov, V V, Ofitserov, V I, and Shval'e, A F. Synthesis of peptide fragments of the hemagglutinin of influenza virus A/Aichi/2/68 (H/sub 3/N/sub 2/). United States: N. p., 1986.
Web.
Samukov, V V, Kalashnikov, V V, Ofitserov, V I, & Shval'e, A F. Synthesis of peptide fragments of the hemagglutinin of influenza virus A/Aichi/2/68 (H/sub 3/N/sub 2/). United States.
Samukov, V V, Kalashnikov, V V, Ofitserov, V I, and Shval'e, A F. 1986.
"Synthesis of peptide fragments of the hemagglutinin of influenza virus A/Aichi/2/68 (H/sub 3/N/sub 2/)". United States.
@article{osti_5244049,
title = {Synthesis of peptide fragments of the hemagglutinin of influenza virus A/Aichi/2/68 (H/sub 3/N/sub 2/)},
author = {Samukov, V V and Kalashnikov, V V and Ofitserov, V I and Shval'e, A F},
abstractNote = {Peptides corresponding to sections 122-133, 136-147, and 154-164 of the heavy chain of the hemagglutinin of influenza virus A/Aichi/2/68 (H/sub 3/N/sub 2/) have been synthesized. The synthesis was performed by the stepwise growth of the peptide chain by means of activated esters of Boc-amino acids and the condensation of peptide blocks by the carbodiimide method in solution. The colored 2-(4-(phenylazo)benylsulfonyl)ethyl group, which can be removed by bases, was used for the protection of the C-terminal carboxy groups of the peptides and peptide blocks, facilitating the purification of the peptides synthesized. After the elimination of the N- and C-terminal carboxy groups of the peptides and peptide blocks, facilitating the purification of the peptides synthesized. After the elimination of the N- and C-terminal protective groups, the peptides were deblocked with 1 M trifluoromethanesulfonic acid in trifluoroacetic acid containing 10% of thioanisole and were purified by prepatative reversed-phase chromatography. Conjugates of the peptides synthesized with bovine serum albumin have been obtained.},
doi = {},
url = {https://www.osti.gov/biblio/5244049},
journal = {Sov. J. Bioorg. Chem. (Engl. Transl.); (United States)},
number = ,
volume = 11:8,
place = {United States},
year = {Sun Jun 01 00:00:00 EDT 1986},
month = {Sun Jun 01 00:00:00 EDT 1986}
}