Inhibition of beta-amino acid transport by diamide does not involve the brush border membrane surface

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Pediatr. Pharmacol.; (United States)

OSTI ID:5243675

Chesney, R W; Gusowski, N; Albright, P

Diamide (dicarboxylic acid bis-(N,N-dimethylamide) has been shown in previous studies to block the uptake of the beta-amino acid taurine at its high affinity transport site in rat renal cortex slices. Diamide may act by increasing the efflux of taurine from the slice. Studies performed in rat slices again indicate enhanced efflux over 8-12 minutes. The time course of reduced glutathione (GSH) depletion from renal cortex is similar, indicating a potential interaction between GSH depletion and inhibition of taurine accumulation. The effect of 9 mM diamide on the Na+ -dependent accumulation of taurine (10 and 250 microM) by brush border membrane vesicles was examined, and the taurine uptake value both initially and at equilibrium was the same in the presence and absence of diamide. Isolation of the brush border surface and subsequent transport studies of taurine are not influenced by diamide. Thus, diamide inhibition of taurine uptake does not involve physiochemical alteration of the membrane surface where active amino acid transport occurs, despite the thiol-oxidizing properties of this agent. Further, these studies suggest that diamide either acts at the basolateral surface, rather than the brush border surface of rat renal cortex or requires the presence of an intact tubule, capable of metabolism, prior to its inhibitory action.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 5243675
• Journal Information: Pediatr. Pharmacol.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMIDES
BIOLOGICAL EFFECTS
TAURINE
MEMBRANE TRANSPORT
AZO COMPOUNDS
CARBON 14 COMPOUNDS
GLUTATHIONE
IN VITRO
INHIBITION
KIDNEYS
RATS
AMINES
ANIMALS
BODY
DRUGS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RODENTS
SULFONIC ACIDS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques