The effect of osmotic stabilizers on the radiometric detection of osmotically sensitive populations of some gram-negative bacteria

Journal Article · Mon Feb 01 00:00:00 EST 1982 · Am. J. Clin. Pathol.; (United States)

DOI:https://doi.org/10.1093/ajcp/77.2.191· OSTI ID:5242684

Martinez, O V; Malinin, T I

The effect of four osmotic stabilizers on the radiometric detection of osmotically sensitive populations of E. coli, S. typhimurium, and E. cloacae was studied. The addition of sucrose, sorbitol, glycerol, or ethylene glycoll to BACTEC 6B blood culture medium failed to improve the sensitivity of the system and produced an inhibitory effect on the level of 14CO2 released by organisms previously exposed to lysozyme and ECTA or to penicillin followed by the lysozyme treatment. The same effect was observed both in blood free media and simulated blood cultures. The addition of proline to sucrose-containing hypertonic media had no effect on growth index readings.

• Research Organization: Department of Surgery, University of Miami School of Medicine
• OSTI ID: 5242684
• Journal Information: Am. J. Clin. Pathol.; (United States), Vol. 77:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ESCHERICHIA COLI
RADIOMETRIC ANALYSIS
GLYCEROL
BIOLOGICAL EFFECTS
GLYCOLS
SACCHAROSE
SALMONELLA TYPHIMURIUM
SORBITOL
CARBON 14 COMPOUNDS
CARBON DIOXIDE
HYPERTONIC SOLUTIONS
OSMOSIS
SENSITIVITY
ALCOHOLS
BACTERIA
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
CHEMICAL ANALYSIS
DIFFUSION
DISACCHARIDES
DISPERSIONS
DIURETICS
DRUGS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MICROORGANISMS
MIXTURES
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
SACCHARIDES
SALMONELLA
SOLUTIONS
550701* - Microbiology- Tracer Techniques