(Investigations into the use of radiolabeled monoclonal antibodies for selective cell labeling in whole blood):
Seventeen monoclonal antibodies (MAbs), 7 specific for human platelets and 10 specific for human polumorphonuclear leukocytes (PMNs) have been evaluated. One MAb has been identified as the antibody most suitable for canine platelets and another has been evaluted as the best among the group, for human neutrophil studies. Indium-111, Tc-99m, and I-125 have been used as the tracers. Six bifunctional chelating agents (BFCAs) were evaluated in order to determine the most efficient agent for maximal cell labeling efficiency. Among these, the DTPA has given us the best results. (4) To botain maximum In-111 chelation and minimum loss of the MAb affinity, the optimal BFCA to MAb ratios for both IgG and IgM type of MAbs were determined. Four different substances, stannous chloride, ascorbic acid, sodium dithionite and sodium borohydride, were evaluated as reducing agents for Tc-99m reduction and its optimal binding to MAbs. Dithionite at the concentration of 200 ug/ml DTPA-MAb solution provides greater than 50% Tc-99m labeling efficiency and maintains its immunospecificity equal to that of In-111-DTPA-MAb. The ability of radiolabeled MAb to interact with blood cells selectively in whole blood and with isolated blood cells was assessed and compared.
- Research Organization:
- Thomas Jefferson Univ., Philadelphia, PA (USA)
- DOE Contract Number:
- FG02-85ER60295
- OSTI ID:
- 5242650
- Report Number(s):
- DOE/ER/60295-3; ON: DE88007058
- Country of Publication:
- United States
- Language:
- English
Similar Records
Investigations into the use of radiolabeled monoclonal antibodies for selective cell labeling in whole blood: Progress report, January 1986-December 1986
Investigations into the use of radiolabeled monoclonal antibodies for selective cell labeling in whole blood; Final report
Related Subjects
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
INDIUM 111
RADIOCHEMISTRY
MONOCLONAL ANTIBODIES
LABELLING
AUTORADIOGRAPHY
BLOOD
BLOOD PLATELETS
DOGS
DTPA
ELECTROPHORESIS
ERYTHROCYTES
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
MAN
PROGRESS REPORT
RABBITS
TECHNETIUM 99
TRANSFERRIN
AMINO ACIDS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHEMISTRY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DOCUMENT TYPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
GLOBULINS-BETA
HOURS LIVING RADIOISOTOPES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
METALLOPROTEINS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
SEPARATION PROCESSES
TECHNETIUM ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
400700 - Radiochemistry & Nuclear Chemistry