Ultraviolet irradiated corneal allografts include antigen scientific unresponsiveness
- Univ. of Texas, Dallas (United States)
The effect of ultraviolet (UV) irradiation on the immunogenicity of corneal allografts was examined in a mouse model. BALB/c corneal allografts were exposed to 200 mJ/cm{sup 2} of UVB irradiation immediately prior to heterotopic transplantation of C57BL/6 recipients. Analysis of cytotoxic T lymphocyte (CTL) and delayed-type hypersensitivity (DTH) responses revealed profound impairment of both forms of cell-mediated immunity. Mice grafted with UVB irradiated corneal grafts 7 days prior to immunization with nonirradiated, immunogenic corneal grafts failed to mount either allospecific CTL or DTH responses. Suppression of DTH responses was cyclophosphamide sensitive; C57BL/6 hosts treated with cyclophosphamide one day prior to receiving UVB irradiated corneal grafts developed normal DTH responses. Allospecific suppression could be transferred to naive recipients using spleen cells from host grafted with UV irradiated corneal allografts. The results indicated that UVB irradiation not only rendered corneal allografts nonimmunogenic but also tolerogenic.
- OSTI ID:
- 5241278
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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