Polyaromatic compounds alter placental protein synthesis in pregnant rats
- Univ. of Florida, Gainesville (United States)
The administration of the polyaromatic compounds {beta}-naphthoflavone ({beta}NF) and 3-methylcholanthrene (3MC) to pregnant rats during mid-gestation has been shown to produce marked feto-placental growth retardation. This study examined secretory protein synthesis in placental tissue from rats following administration of {beta}NF on gestation days (gd) 11-14 or 3MC on gd 12-14. Explants of placental basal zone tissue were cultured for 24 hours in serum-free medium in the presence of ({sup 3}H)leucine. Secreted proteins were analyzed by two-dimensional SDS-polyacrylamide gel electrophoresis followed by either fluorography or immunostaining. Total incorporation of ({sup 3}H)leucine into secreted proteins was not altered in BZ explants from {beta}NF or 3MC-treated animals. However a selective decrease was observed in ({sup 3}H)leucine incorporation into a major complex of proteins with apparent molecular weight of 25-30,000 and isoelectric point between 5.3 to 5.7. This group of proteins has been further identified as being related to rat pituitary growth hormone (GH) using N-terminal amino acid microsequencing of individual spots from 2-D SDS-PA gels. This is the first report that synthesis of GH-related proteins by rat placenta is decreased following {beta}NF and 3MC administration, a change which may underlie the feto-placental growth retardation associated with these polyaromatic compounds.
- OSTI ID:
- 5238724
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
POLYCYCLIC AROMATIC HYDROCARBONS
BIOLOGICAL EFFECTS
PROTEINS
BIOSYNTHESIS
3-METHYLCHOLANTHRENE
AMINO ACID SEQUENCE
ELECTROPHORESIS
INHIBITION
LEUCINE
PLACENTA
PREGNANCY
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
AROMATICS
CARBOXYLIC ACIDS
CONDENSED AROMATICS
FETAL MEMBRANES
HYDROCARBONS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MEMBRANES
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
RODENTS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology